frap data analyses Search Results


fraction collector  (GE Healthcare)
95
GE Healthcare fraction collector
Fraction Collector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fraction collector/product/GE Healthcare
Average 95 stars, based on 1 article reviews
fraction collector - by Bioz Stars, 2026-06
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zen software blue edition  (Carl Zeiss)
90
Carl Zeiss zen software blue edition
Zen Software Blue Edition, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zen software blue edition/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
zen software blue edition - by Bioz Stars, 2026-06
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frap recovery data analysis  (GraphPad Software Inc)
90
GraphPad Software Inc frap recovery data analysis
Frap Recovery Data Analysis, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/frap recovery data analysis/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
frap recovery data analysis - by Bioz Stars, 2026-06
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frap module  (Carl Zeiss)
94
Carl Zeiss frap module
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Frap Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/frap module/product/Carl Zeiss
Average 94 stars, based on 1 article reviews
frap module - by Bioz Stars, 2026-06
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image-analysis software metamorph discovery-1  (MetaMorph Inc)
90
MetaMorph Inc image-analysis software metamorph discovery-1
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Image Analysis Software Metamorph Discovery 1, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/image-analysis software metamorph discovery-1/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
image-analysis software metamorph discovery-1 - by Bioz Stars, 2026-06
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frap data harmony 3 5 1  (Revvity)
94
Revvity frap data harmony 3 5 1
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Frap Data Harmony 3 5 1, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/frap data harmony 3 5 1/product/Revvity
Average 94 stars, based on 1 article reviews
frap data harmony 3 5 1 - by Bioz Stars, 2026-06
94/100 stars
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prism software v5.01  (GraphPad Software Inc)
90
GraphPad Software Inc prism software v5.01
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Prism Software V5.01, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism software v5.01/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism software v5.01 - by Bioz Stars, 2026-06
90/100 stars
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frap plugin  (Carl Zeiss)
90
Carl Zeiss frap plugin
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Frap Plugin, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/frap plugin/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
frap plugin - by Bioz Stars, 2026-06
90/100 stars
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lsm 510 nlo system  (Carl Zeiss)
90
Carl Zeiss lsm 510 nlo system
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Lsm 510 Nlo System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lsm 510 nlo system/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
lsm 510 nlo system - by Bioz Stars, 2026-06
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frequency response analysis (fra) method metrohm autolab  (Metrohm AG)
90
Metrohm AG frequency response analysis (fra) method metrohm autolab
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Frequency Response Analysis (Fra) Method Metrohm Autolab, supplied by Metrohm AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/frequency response analysis (fra) method metrohm autolab/product/Metrohm AG
Average 90 stars, based on 1 article reviews
frequency response analysis (fra) method metrohm autolab - by Bioz Stars, 2026-06
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eco chemie frequency response analyzer (fra) 4.9 software  (Chemie GmbH)
90
Chemie GmbH eco chemie frequency response analyzer (fra) 4.9 software
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Eco Chemie Frequency Response Analyzer (Fra) 4.9 Software, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eco chemie frequency response analyzer (fra) 4.9 software/product/Chemie GmbH
Average 90 stars, based on 1 article reviews
eco chemie frequency response analyzer (fra) 4.9 software - by Bioz Stars, 2026-06
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frap data analysis  (Nikon)
99
Nikon frap data analysis
a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching <t>(FRAP)</t> of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS <t>X</t> <t>software.</t> Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.
Frap Data Analysis, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/frap data analysis/product/Nikon
Average 99 stars, based on 1 article reviews
frap data analysis - by Bioz Stars, 2026-06
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Image Search Results


a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching (FRAP) of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS X software. Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.

Journal: Nature Communications

Article Title: Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing

doi: 10.1038/s41467-022-31220-8

Figure Lengend Snippet: a Top: schematic diagram of Cry2-based optogenetic constructs of WASP and its N-terminal (NT) and C-terminal (CT) IDRs (created with BioRender.com). The NT and CT locations are shown on the top of PONDR IDR prediction diagram. Bottom: time-lapse confocal images of HEK293 cells expressing photo-activated optogenetic constructs of WASP, NT, or CT following photoactivation. Scale bar = 2 µm. The experiment was repeated at least three times independently. b Confocal imaging of fusion of optoWASP optogenetic droplets after blue light activation. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. c Confocal images showing fluorescence recovery after photobleaching (FRAP) of optoWASP droplets. Time-lapse images of the region framed in the box are shown below. Scale bar = 2 µm. The experiment was repeated at least three times independently. d Representative single-plane confocal images and colocalization analysis of optoWASP droplets (N-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson correlation coefficient of colocalization (Pearson’s r ) was calculated using the Leica LAS X software. Analyzed image n = 17, data are shown as mean ± SD. e Representative single-plane confocal images and colocalization analysis of optoWASP droplets (C-terminal WASP) with endogenous SRSF2 and nascent RNA. Insets show the framed region in the images. Scale bar = 2 µm. The Pearson’s r was calculated using the Leica LAS X software. Analyzed image n = 15, data are shown as mean ± SD.

Article Snippet: After image acquisition, the data were analyzed using FRAP module installed in ZEISS software.

Techniques: Construct, Expressing, Imaging, Activation Assay, Fluorescence, Software